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A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses

Loop-mediated isothermal amplification (LAMP) has been widely used in the detection of pathogens causing infectious diseases. However, mismatches between primers (especially in the 3′-end) and templates significantly reduced the amplification efficiency of LAMP, and limited its application to geneti...

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Detalles Bibliográficos
Autores principales: Zhou, Yi, Wan, Zhenzhou, Yang, Shuting, Li, Yingxue, Li, Min, Wang, Binghui, Hu, Yihong, Xia, Xueshan, Jin, Xia, Yu, Na, Zhang, Chiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6518337/
https://www.ncbi.nlm.nih.gov/pubmed/31139171
http://dx.doi.org/10.3389/fmicb.2019.01056