Cargando…

Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactionsby Differential Affinity Immobilization

Long Interspersed Nuclear Element-1 (LINE-1, L1) constitutes a family of autonomous, self-replicating genetic elements known as retrotransposons. Although most are inactive, copious L1 sequences populate the human genome. L1s proliferate in a ‘copy-and-paste’ fashion through an RNA intermediate; a f...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jiang, Hua, Taylor, Martin S., Molloy, Kelly R., Altukhov, Ilya, LaCava, John
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2019
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519465/ https://www.ncbi.nlm.nih.gov/pubmed/31106238 http://dx.doi.org/10.21769/BioProtoc.3200

Ejemplares similares

Dissection of affinity captured LINE-1 macromolecular complexes
por: Taylor, Martin S, et al.
Publicado: (2018)

Affinity proteomic dissection of the human nuclear cap-binding complex interactome
por: Dou, Yuhui, et al.
Publicado: (2020)

Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes
por: Fritz, Sarah E., et al.
Publicado: (2018)

Computational prediction of lncRNA-mRNA interactionsby integrating tissue specificity in human transcriptome
por: Iwakiri, Junichi, et al.
Publicado: (2017)

Purification and analysis of endogenous human RNA exosome complexes
por: Domanski, Michal, et al.
Publicado: (2016)

Ribonucleoprotein purification and characterization using RNA Mango
por: Panchapakesan, Shanker Shyam S., et al.
Publicado: (2017)

Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP
por: Perederina, Anna, et al.
Publicado: (2020)

A high-throughput pipeline for the production of synthetic antibodies for analysis of ribonucleoprotein complexes
por: Na, Hong, et al.
Publicado: (2016)

Protein Complex Affinity Capture from Cryomilled Mammalian Cells
por: LaCava, John, et al.
Publicado: (2016)

LINE-1 ORF2p expression is nearly imperceptible in human cancers
por: Ardeljan, Daniel, et al.
Publicado: (2019)

Low-input RNase footprinting for simultaneous quantification of cytosolic and mitochondrial translation
por: Li, Qianru, et al.
Publicado: (2022)

Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull
por: Rodgers, Margaret L., et al.
Publicado: (2015)

RNase H-based analysis of synthetic mRNA 5′ cap incorporation
por: Chan, S. Hong, et al.
Publicado: (2022)

An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes
por: Taoka, Masato, et al.
Publicado: (2009)

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
por: Kim, Sojung, et al.
Publicado: (2014)

High-salt–recovered sequences are associated with the active chromosomal compartment and with large ribonucleoprotein complexes including nuclear bodies
por: Baudement, Marie-Odile, et al.
Publicado: (2018)

Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
por: Serruya, Raphael, et al.
Publicado: (2015)

RNase L promotes the formation of unique ribonucleoprotein granules distinct from stress granules
por: Burke, James M., et al.
Publicado: (2020)

RNase-mediated protein footprint sequencing reveals protein-binding sites throughout the human transcriptome
por: Silverman, Ian M, et al.
Publicado: (2014)

Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
por: Kühn-Hölsken, Eva, et al.
Publicado: (2007)

Dissecting functional cooperation among protein subunits in archaeal RNase P, a catalytic ribonucleoprotein complex
por: Chen, Wen-Yi, et al.
Publicado: (2010)

Elucidation of structure–function relationships in Methanocaldococcus jannaschii RNase P, a multi-subunit catalytic ribonucleoprotein
por: Phan, Hong-Duc, et al.
Publicado: (2022)

An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins
por: Leppek, Kathrin, et al.
Publicado: (2014)

Argonaute-based programmable RNase as a tool for cleavage of highly-structured RNA
por: Dayeh, Daniel M, et al.
Publicado: (2018)

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
por: Liu, Qizhi, et al.
Publicado: (2018)

Characterization of LINE-1 Ribonucleoprotein Particles
por: Doucet, Aurélien J., et al.
Publicado: (2010)

Expression and detection of LINE-1 ORF-encoded proteins
por: Dai, Lixin, et al.
Publicado: (2014)

Improvement of RNA secondary structure prediction using RNase H cleavage and randomized oligonucleotides
por: Kauffmann, Andrew D., et al.
Publicado: (2009)

Defining the 5΄ and 3΄ landscape of the Drosophila transcriptome with Exo-seq and RNaseH-seq
por: Afik, Shaked, et al.
Publicado: (2017)

Human RNase 4 improves mRNA sequence characterization by LC–MS/MS
por: Wolf, Eric J, et al.
Publicado: (2022)

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery
por: Gaj, Thomas, et al.
Publicado: (2017)

Sensitive and label-free biosensing of RNA with predicted secondary structures by a triplex affinity capture method
por: Carrascosa, Laura G., et al.
Publicado: (2012)

Purification of synchronized Escherichia coli transcription elongation complexes by reversible immobilization on magnetic beads
por: Kelly, Skyler L., et al.
Publicado: (2022)

Mechanistic and Structural Studies of Protein-Only RNase P Compared to Ribonucleoproteins Reveal the Two Faces of the Same Enzymatic Activity
por: Schelcher, Cédric, et al.
Publicado: (2016)

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing
por: Lyu, Pin, et al.
Publicado: (2019)

Large-scale Purification of Type III Toxin-antitoxin Ribonucleoprotein Complex and its Components from Escherichia coli for Biophysical Studies
por: Manikandan, Parthasarathy, et al.
Publicado: (2023)

Computational Assessment of Protein–protein Binding Affinity by Reversely Engineering the Energetics in Protein Complexes
por: Wang, Bo, et al.
Publicado: (2021)

Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ
por: Wang, Shengliu, et al.
Publicado: (2017)

Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags
por: Salvail-Lacoste, Alix, et al.
Publicado: (2013)

Clinical drug response can be predicted using baseline gene expression levels and in vitro drug sensitivity in cell lines
por: Geeleher, Paul, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_gkhnf4aiu5jipil1jphecrsilt