CRISPR/Cas9-facilitated engineering with growth-coupled and sensor-guided in vivo screening of enzyme variants for a more efficient chorismate pathway in E. coli

Protein engineering plays an increasingly important role in developing new and optimizing existing metabolic pathways for biosynthesis. Conventional screening approach of libraries of gene and enzyme variants is often done using a host strain under conditions not relevant to the cultivation or intra...

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Detalles Bibliográficos
Autores principales: Chen, Minliang, Chen, Lin, Zeng, An-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520568/
https://www.ncbi.nlm.nih.gov/pubmed/31193188
http://dx.doi.org/10.1016/j.mec.2019.e00094

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520568/
https://www.ncbi.nlm.nih.gov/pubmed/31193188
http://dx.doi.org/10.1016/j.mec.2019.e00094

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