Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry fluorescence selection cassette were delivered in tandem via homology-directed repair t...

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Detalles Bibliográficos
Autores principales: Roberts, Brock, Hendershott, Melissa C., Arakaki, Joy, Gerbin, Kaytlyn A., Malik, Haseeb, Nelson, Angelique, Gehring, Jamie, Hookway, Caroline, Ludmann, Susan A., Yang, Ruian, Haupt, Amanda, Grancharova, Tanya, Valencia, Veronica, Fuqua, Margaret A., Tucker, Andrew, Rafelski, Susanne M., Gunawardane, Ruwanthi N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522946/
https://www.ncbi.nlm.nih.gov/pubmed/30956114
http://dx.doi.org/10.1016/j.stemcr.2019.03.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522946/
https://www.ncbi.nlm.nih.gov/pubmed/30956114
http://dx.doi.org/10.1016/j.stemcr.2019.03.001

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