Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay

We investigated the contribution of host immune cells to bacterial killing in a whole-blood bactericidal activity (WBA) assay, an ex vivo model used to test efficacy of drugs against mycobacterium tuberculosis (Mtb). We performed WBA assays with immuno-magnetic depletion of specific cell types, in t...

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Detalles Bibliográficos
Autores principales: Cross, Gail B., Yeo, Benjamin C-M, Hutchinson, Paul Edward, Tan, Mark C., Verma, Rupangi, Lu, Qingshu, Paton, Nicholas I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524797/
https://www.ncbi.nlm.nih.gov/pubmed/31100071
http://dx.doi.org/10.1371/journal.pone.0216616
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author Cross, Gail B.
Yeo, Benjamin C-M
Hutchinson, Paul Edward
Tan, Mark C.
Verma, Rupangi
Lu, Qingshu
Paton, Nicholas I.
author_facet Cross, Gail B.
Yeo, Benjamin C-M
Hutchinson, Paul Edward
Tan, Mark C.
Verma, Rupangi
Lu, Qingshu
Paton, Nicholas I.
author_sort Cross, Gail B.
collection PubMed
description We investigated the contribution of host immune cells to bacterial killing in a whole-blood bactericidal activity (WBA) assay, an ex vivo model used to test efficacy of drugs against mycobacterium tuberculosis (Mtb). We performed WBA assays with immuno-magnetic depletion of specific cell types, in the presence or absence of rifampicin. Innate immune cells decreased Mtb growth in absence of drug, but appeared to diminish the cidal activity of rifampicin, possibly attributable to intracellular bacterial sequestration. Adaptive immune cells had no effect with or without drug. The WBA assay may have potential for testing adjunctive host-directed therapies acting on phagocytic cells.
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spelling pubmed-65247972019-05-31 Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay Cross, Gail B. Yeo, Benjamin C-M Hutchinson, Paul Edward Tan, Mark C. Verma, Rupangi Lu, Qingshu Paton, Nicholas I. PLoS One Research Article We investigated the contribution of host immune cells to bacterial killing in a whole-blood bactericidal activity (WBA) assay, an ex vivo model used to test efficacy of drugs against mycobacterium tuberculosis (Mtb). We performed WBA assays with immuno-magnetic depletion of specific cell types, in the presence or absence of rifampicin. Innate immune cells decreased Mtb growth in absence of drug, but appeared to diminish the cidal activity of rifampicin, possibly attributable to intracellular bacterial sequestration. Adaptive immune cells had no effect with or without drug. The WBA assay may have potential for testing adjunctive host-directed therapies acting on phagocytic cells. Public Library of Science 2019-05-17 /pmc/articles/PMC6524797/ /pubmed/31100071 http://dx.doi.org/10.1371/journal.pone.0216616 Text en © 2019 Cross et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cross, Gail B.
Yeo, Benjamin C-M
Hutchinson, Paul Edward
Tan, Mark C.
Verma, Rupangi
Lu, Qingshu
Paton, Nicholas I.
Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay
title Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay
title_full Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay
title_fullStr Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay
title_full_unstemmed Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay
title_short Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay
title_sort impact of selective immune-cell depletion on growth of mycobacterium tuberculosis (mtb) in a whole-blood bactericidal activity (wba) assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524797/
https://www.ncbi.nlm.nih.gov/pubmed/31100071
http://dx.doi.org/10.1371/journal.pone.0216616
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