Cargando…
6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda
A long-standing question in the field of embryogenesis is how the zygotic genome is precisely activated by maternal factors, allowing normal early embryonic development. We have previously shown that N6-methyladenine (6mA) DNA modification is highly dynamic in early Drosophila embryos and forms an e...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525185/ https://www.ncbi.nlm.nih.gov/pubmed/31101825 http://dx.doi.org/10.1038/s41467-019-10202-3 |
_version_ | 1783419673175392256 |
---|---|
author | He, Shunmin Zhang, Guoqiang Wang, Jiajia Gao, Yajie Sun, Ruidi Cao, Zhijie Chen, Zhenping Zheng, Xiudeng Yuan, Jiao Luo, Yuewan Wang, Xiaona Zhang, Wenxin Zhang, Peng Zhao, Yi He, Chuan Tao, Yi Sun, Qinmiao Chen, Dahua |
author_facet | He, Shunmin Zhang, Guoqiang Wang, Jiajia Gao, Yajie Sun, Ruidi Cao, Zhijie Chen, Zhenping Zheng, Xiudeng Yuan, Jiao Luo, Yuewan Wang, Xiaona Zhang, Wenxin Zhang, Peng Zhao, Yi He, Chuan Tao, Yi Sun, Qinmiao Chen, Dahua |
author_sort | He, Shunmin |
collection | PubMed |
description | A long-standing question in the field of embryogenesis is how the zygotic genome is precisely activated by maternal factors, allowing normal early embryonic development. We have previously shown that N6-methyladenine (6mA) DNA modification is highly dynamic in early Drosophila embryos and forms an epigenetic mark. However, little is known about how 6mA-formed epigenetic information is decoded. Here we report that the Fox-family protein Jumu binds 6mA-marked DNA and acts as a maternal factor to regulate the maternal-to-zygotic transition. We find that zelda encoding the pioneer factor Zelda is marked by 6mA. Our genetic assays suggest that Jumu controls the proper zygotic genome activation (ZGA) in early embryos, at least in part, by regulating zelda expression. Thus, our findings not only support that the 6mA-formed epigenetic marks can be read by specific transcription factors, but also uncover a mechanism by which the Jumu regulates ZGA partially through Zelda in early embryos. |
format | Online Article Text |
id | pubmed-6525185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65251852019-05-20 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda He, Shunmin Zhang, Guoqiang Wang, Jiajia Gao, Yajie Sun, Ruidi Cao, Zhijie Chen, Zhenping Zheng, Xiudeng Yuan, Jiao Luo, Yuewan Wang, Xiaona Zhang, Wenxin Zhang, Peng Zhao, Yi He, Chuan Tao, Yi Sun, Qinmiao Chen, Dahua Nat Commun Article A long-standing question in the field of embryogenesis is how the zygotic genome is precisely activated by maternal factors, allowing normal early embryonic development. We have previously shown that N6-methyladenine (6mA) DNA modification is highly dynamic in early Drosophila embryos and forms an epigenetic mark. However, little is known about how 6mA-formed epigenetic information is decoded. Here we report that the Fox-family protein Jumu binds 6mA-marked DNA and acts as a maternal factor to regulate the maternal-to-zygotic transition. We find that zelda encoding the pioneer factor Zelda is marked by 6mA. Our genetic assays suggest that Jumu controls the proper zygotic genome activation (ZGA) in early embryos, at least in part, by regulating zelda expression. Thus, our findings not only support that the 6mA-formed epigenetic marks can be read by specific transcription factors, but also uncover a mechanism by which the Jumu regulates ZGA partially through Zelda in early embryos. Nature Publishing Group UK 2019-05-17 /pmc/articles/PMC6525185/ /pubmed/31101825 http://dx.doi.org/10.1038/s41467-019-10202-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article He, Shunmin Zhang, Guoqiang Wang, Jiajia Gao, Yajie Sun, Ruidi Cao, Zhijie Chen, Zhenping Zheng, Xiudeng Yuan, Jiao Luo, Yuewan Wang, Xiaona Zhang, Wenxin Zhang, Peng Zhao, Yi He, Chuan Tao, Yi Sun, Qinmiao Chen, Dahua 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
title | 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
title_full | 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
title_fullStr | 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
title_full_unstemmed | 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
title_short | 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
title_sort | 6ma-dna-binding factor jumu controls maternal-to-zygotic transition upstream of zelda |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525185/ https://www.ncbi.nlm.nih.gov/pubmed/31101825 http://dx.doi.org/10.1038/s41467-019-10202-3 |
work_keys_str_mv | AT heshunmin 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT zhangguoqiang 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT wangjiajia 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT gaoyajie 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT sunruidi 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT caozhijie 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT chenzhenping 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT zhengxiudeng 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT yuanjiao 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT luoyuewan 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT wangxiaona 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT zhangwenxin 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT zhangpeng 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT zhaoyi 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT hechuan 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT taoyi 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT sunqinmiao 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda AT chendahua 6madnabindingfactorjumucontrolsmaternaltozygotictransitionupstreamofzelda |