A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies

Cas9 is a site-specific RNA-guided endonuclease (RGEN) that can be used for precise genome editing in various cell types from multiple species. Ribonucleoprotein (RNP) complexes, which contains the Cas9 protein in complex with a guide RNA, are sufficient for the precise editing of genomes in various...

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Detalles Bibliográficos
Autores principales: Rajagopalan, Nandhakishore, Kagale, Sateesh, Bhowmik, Pankaj, Song, Halim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526429/
https://www.ncbi.nlm.nih.gov/pubmed/31164560
http://dx.doi.org/10.3390/mps1020017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526429/
https://www.ncbi.nlm.nih.gov/pubmed/31164560
http://dx.doi.org/10.3390/mps1020017

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