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On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development

NIR spectroscopy is a non-destructive tool for in-situ, on-line bioprocess monitoring. One of its most frequent applications is the determination of metabolites during cultivation, especially glucose. Previous studies have usually investigated the applicability of Near Infrared (NIR) spectroscopy at...

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Autores principales: Kozma, Bence, Salgó, András, Gergely, Szilveszter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527534/
https://www.ncbi.nlm.nih.gov/pubmed/30806782
http://dx.doi.org/10.1007/s00449-019-02091-z
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author Kozma, Bence
Salgó, András
Gergely, Szilveszter
author_facet Kozma, Bence
Salgó, András
Gergely, Szilveszter
author_sort Kozma, Bence
collection PubMed
description NIR spectroscopy is a non-destructive tool for in-situ, on-line bioprocess monitoring. One of its most frequent applications is the determination of metabolites during cultivation, especially glucose. Previous studies have usually investigated the applicability of Near Infrared (NIR) spectroscopy at one bioreactor scale but the effect of scale up was not explored. In this study, the complete scale up from shake flask (1 L) through 20 L, 100 L and 1000 L up to 5000 L bioreactor volume level was monitored with on-line NIR spectroscopy. The differences between runs and scales were examined using principal component analysis. The bioreactor runs were relatively similar regardless of scales but the shake flasks differed strongly from bioreactor runs. The glucose concentration throughout five 5000 L scale bioreactor runs were predicted by partial least squares regression models that were based on pre-processed spectra of bioreactor runs and combinations of them. The model that produced the lowest error of prediction (4.18 mM on a 29 mM concentration range) for all five runs in the prediction set was based on the combination of 20 L and 100 L data. This result demonstrated the capabilities and the limitations of an NIR system for glucose monitoring in mammalian cell cultivations.
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spelling pubmed-65275342019-06-07 On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development Kozma, Bence Salgó, András Gergely, Szilveszter Bioprocess Biosyst Eng Research Paper NIR spectroscopy is a non-destructive tool for in-situ, on-line bioprocess monitoring. One of its most frequent applications is the determination of metabolites during cultivation, especially glucose. Previous studies have usually investigated the applicability of Near Infrared (NIR) spectroscopy at one bioreactor scale but the effect of scale up was not explored. In this study, the complete scale up from shake flask (1 L) through 20 L, 100 L and 1000 L up to 5000 L bioreactor volume level was monitored with on-line NIR spectroscopy. The differences between runs and scales were examined using principal component analysis. The bioreactor runs were relatively similar regardless of scales but the shake flasks differed strongly from bioreactor runs. The glucose concentration throughout five 5000 L scale bioreactor runs were predicted by partial least squares regression models that were based on pre-processed spectra of bioreactor runs and combinations of them. The model that produced the lowest error of prediction (4.18 mM on a 29 mM concentration range) for all five runs in the prediction set was based on the combination of 20 L and 100 L data. This result demonstrated the capabilities and the limitations of an NIR system for glucose monitoring in mammalian cell cultivations. Springer Berlin Heidelberg 2019-02-26 2019 /pmc/articles/PMC6527534/ /pubmed/30806782 http://dx.doi.org/10.1007/s00449-019-02091-z Text en © The Author(s) 2019 OpenAccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Paper
Kozma, Bence
Salgó, András
Gergely, Szilveszter
On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
title On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
title_full On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
title_fullStr On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
title_full_unstemmed On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
title_short On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
title_sort on-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527534/
https://www.ncbi.nlm.nih.gov/pubmed/30806782
http://dx.doi.org/10.1007/s00449-019-02091-z
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