Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection

BACKGROUND: CRISPR/Cas9 mediated gene knockout is a powerful tool for genome editing with the ability to target multiple genes simultaneously. Establishing an efficient, multiplexed gene knockout system using CRISPR/Cas9 that is both simple and robust in its application would further advance the ado...

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Detalles Bibliográficos
Autores principales: Khan, Fehad J., Yuen, Garmen, Luo, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528186/
https://www.ncbi.nlm.nih.gov/pubmed/31139343
http://dx.doi.org/10.1186/s13578-019-0304-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528186/
https://www.ncbi.nlm.nih.gov/pubmed/31139343
http://dx.doi.org/10.1186/s13578-019-0304-0

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