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Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
BACKGROUND: CRISPR/Cas9 mediated gene knockout is a powerful tool for genome editing with the ability to target multiple genes simultaneously. Establishing an efficient, multiplexed gene knockout system using CRISPR/Cas9 that is both simple and robust in its application would further advance the ado...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528186/ https://www.ncbi.nlm.nih.gov/pubmed/31139343 http://dx.doi.org/10.1186/s13578-019-0304-0 |