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Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method
OBJECTIVE(S): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. MATERIALS AND METHODS: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528719/ https://www.ncbi.nlm.nih.gov/pubmed/31156791 http://dx.doi.org/10.22038/ijbms.2019.31611.7611 |
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author | Lin, Yiping Wei, Yanli Hu, Xiaoxia Wu, Meiling Yao, Jingchan Ying, Xiaoqian Fu, Xiaoyan Ding, Mingxing Qiao, Liman |
author_facet | Lin, Yiping Wei, Yanli Hu, Xiaoxia Wu, Meiling Yao, Jingchan Ying, Xiaoqian Fu, Xiaoyan Ding, Mingxing Qiao, Liman |
author_sort | Lin, Yiping |
collection | PubMed |
description | OBJECTIVE(S): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. MATERIALS AND METHODS: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. RESULTS: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. CONCLUSION: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure. |
format | Online Article Text |
id | pubmed-6528719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-65287192019-05-31 Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method Lin, Yiping Wei, Yanli Hu, Xiaoxia Wu, Meiling Yao, Jingchan Ying, Xiaoqian Fu, Xiaoyan Ding, Mingxing Qiao, Liman Iran J Basic Med Sci Original Article OBJECTIVE(S): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. MATERIALS AND METHODS: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. RESULTS: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. CONCLUSION: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure. Mashhad University of Medical Sciences 2019-03 /pmc/articles/PMC6528719/ /pubmed/31156791 http://dx.doi.org/10.22038/ijbms.2019.31611.7611 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lin, Yiping Wei, Yanli Hu, Xiaoxia Wu, Meiling Yao, Jingchan Ying, Xiaoqian Fu, Xiaoyan Ding, Mingxing Qiao, Liman Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method |
title | Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method |
title_full | Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method |
title_fullStr | Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method |
title_full_unstemmed | Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method |
title_short | Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method |
title_sort | evaluation of lentinan effects on cytochrome p450 activity in rats by a cocktail method |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528719/ https://www.ncbi.nlm.nih.gov/pubmed/31156791 http://dx.doi.org/10.22038/ijbms.2019.31611.7611 |
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