High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins

Efficient gene deletion methods are essential for the high-throughput study of gene function. Compared to most ascomycete model systems, gene deletion is more laborious in mushroom-forming basidiomycetes due to the relatively low incidence of homologous recombination (HR) and relatively high inciden...

Descripción completa

Detalles Bibliográficos
Autores principales: Jan Vonk, Peter, Escobar, Natalia, Wösten, Han A. B., Lugones, Luis G., Ohm, Robin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529522/
https://www.ncbi.nlm.nih.gov/pubmed/31113995
http://dx.doi.org/10.1038/s41598-019-44133-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529522/
https://www.ncbi.nlm.nih.gov/pubmed/31113995
http://dx.doi.org/10.1038/s41598-019-44133-2

Ejemplares similares