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Non-canonical amino acid labeling in proteomics and biotechnology

Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries. Labeled proteins can then be selectively conjugated to fluorophores, aff...

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Detalles Bibliográficos
Autores principales: Saleh, Aya M., Wilding, Kristen M., Calve, Sarah, Bundy, Bradley C., Kinzer-Ursem, Tamara L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529998/
https://www.ncbi.nlm.nih.gov/pubmed/31139251
http://dx.doi.org/10.1186/s13036-019-0166-3