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In situ fixation and subsequent collection of cultured endothelial cells in a shear flow

In situ fixation of adherent cells is a necessary process for downstream assays. Current methods to dissociate adherent endothelial cells require the use of a cell scraper that may introduce variability in nuclear morphology. Also, a cell scraper is not an option for experiments using sealed flow ch...

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Detalles Bibliográficos
Autores principales: Aldrich, Jessica L., Long, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531827/
https://www.ncbi.nlm.nih.gov/pubmed/31193472
http://dx.doi.org/10.1016/j.mex.2019.05.001