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In situ fixation and subsequent collection of cultured endothelial cells in a shear flow
In situ fixation of adherent cells is a necessary process for downstream assays. Current methods to dissociate adherent endothelial cells require the use of a cell scraper that may introduce variability in nuclear morphology. Also, a cell scraper is not an option for experiments using sealed flow ch...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531827/ https://www.ncbi.nlm.nih.gov/pubmed/31193472 http://dx.doi.org/10.1016/j.mex.2019.05.001 |