A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

BACKGROUND: Intrabodies enable targeting of proteins in live cells, but generating specific intrabodies against the thousands of proteins in a proteome poses a challenge. We leverage the widespread availability of fluorescently labelled proteins to visualize and manipulate intracellular signalling p...

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Detalles Bibliográficos
Autores principales: Prole, David L., Taylor, Colin W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6533734/
https://www.ncbi.nlm.nih.gov/pubmed/31122229
http://dx.doi.org/10.1186/s12915-019-0662-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6533734/
https://www.ncbi.nlm.nih.gov/pubmed/31122229
http://dx.doi.org/10.1186/s12915-019-0662-4

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