Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells

We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore proteins (KPs) in live budding yeast cells. The low copy number of many KPs an...

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Detalles Bibliográficos
Autores principales: Guo, Wenjun, Kumar, Sunil, Görlitz, Frederik, Garcia, Edwin, Alexandrov, Yuriy, Munro, Ian, Kelly, Douglas J., Warren, Sean, Thorpe, Peter, Dunsby, Christopher, French, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537140/
https://www.ncbi.nlm.nih.gov/pubmed/30629461
http://dx.doi.org/10.1177/2472630318819240

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537140/
https://www.ncbi.nlm.nih.gov/pubmed/30629461
http://dx.doi.org/10.1177/2472630318819240

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