Improved TGIRT-seq methods for comprehensive transcriptome profiling with decreased adapter dimer formation and bias correction

Thermostable group II intron reverse transcriptases (TGIRTs) with high fidelity and processivity have been used for a variety of RNA sequencing (RNA-seq) applications, including comprehensive profiling of whole-cell, exosomal, and human plasma RNAs; quantitative tRNA-seq based on the ability of TGIR...

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Detalles Bibliográficos
Autores principales: Xu, Hengyi, Yao, Jun, Wu, Douglas C., Lambowitz, Alan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538698/
https://www.ncbi.nlm.nih.gov/pubmed/31138886
http://dx.doi.org/10.1038/s41598-019-44457-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538698/
https://www.ncbi.nlm.nih.gov/pubmed/31138886
http://dx.doi.org/10.1038/s41598-019-44457-z

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