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Metal Ions Activate the Human Taste Receptor TAS2R7
Divalent and trivalent salts exhibit a complex taste profile. They are perceived as being astringent/drying, sour, bitter, and metallic. We hypothesized that human bitter-taste receptors may mediate some taste attributes of these salts. Using a cell-based functional assay, we found that TAS2R7 respo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538953/ https://www.ncbi.nlm.nih.gov/pubmed/31066447 http://dx.doi.org/10.1093/chemse/bjz024 |
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author | Wang, Yi Zajac, Amanda L Lei, Weiwei Christensen, Carol M Margolskee, Robert F Bouysset, Cédric Golebiowski, Jérôme Zhao, Huabin Fiorucci, Sébastien Jiang, Peihua |
author_facet | Wang, Yi Zajac, Amanda L Lei, Weiwei Christensen, Carol M Margolskee, Robert F Bouysset, Cédric Golebiowski, Jérôme Zhao, Huabin Fiorucci, Sébastien Jiang, Peihua |
author_sort | Wang, Yi |
collection | PubMed |
description | Divalent and trivalent salts exhibit a complex taste profile. They are perceived as being astringent/drying, sour, bitter, and metallic. We hypothesized that human bitter-taste receptors may mediate some taste attributes of these salts. Using a cell-based functional assay, we found that TAS2R7 responds to a broad range of divalent and trivalent salts, including zinc, calcium, magnesium, copper, manganese, and aluminum, but not to potassium, suggesting TAS2R7 may act as a metal cation receptor mediating bitterness of divalent and trivalent salts. Molecular modeling and mutagenesis analysis identified 2 residues, H94(3.37) and E264(7.32), in TAS2R7 that appear to be responsible for the interaction of TAS2R7 with metallic ions. Taste receptors are found in both oral and extraoral tissues. The responsiveness of TAS2R7 to various mineral salts suggests it may act as a broad sensor, similar to the calcium-sensing receptor, for biologically relevant metal cations in both oral and extraoral tissues. |
format | Online Article Text |
id | pubmed-6538953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-65389532019-06-11 Metal Ions Activate the Human Taste Receptor TAS2R7 Wang, Yi Zajac, Amanda L Lei, Weiwei Christensen, Carol M Margolskee, Robert F Bouysset, Cédric Golebiowski, Jérôme Zhao, Huabin Fiorucci, Sébastien Jiang, Peihua Chem Senses Original Articles Divalent and trivalent salts exhibit a complex taste profile. They are perceived as being astringent/drying, sour, bitter, and metallic. We hypothesized that human bitter-taste receptors may mediate some taste attributes of these salts. Using a cell-based functional assay, we found that TAS2R7 responds to a broad range of divalent and trivalent salts, including zinc, calcium, magnesium, copper, manganese, and aluminum, but not to potassium, suggesting TAS2R7 may act as a metal cation receptor mediating bitterness of divalent and trivalent salts. Molecular modeling and mutagenesis analysis identified 2 residues, H94(3.37) and E264(7.32), in TAS2R7 that appear to be responsible for the interaction of TAS2R7 with metallic ions. Taste receptors are found in both oral and extraoral tissues. The responsiveness of TAS2R7 to various mineral salts suggests it may act as a broad sensor, similar to the calcium-sensing receptor, for biologically relevant metal cations in both oral and extraoral tissues. Oxford University Press 2019-05 2019-04-23 /pmc/articles/PMC6538953/ /pubmed/31066447 http://dx.doi.org/10.1093/chemse/bjz024 Text en © The Author(s) 2019. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Wang, Yi Zajac, Amanda L Lei, Weiwei Christensen, Carol M Margolskee, Robert F Bouysset, Cédric Golebiowski, Jérôme Zhao, Huabin Fiorucci, Sébastien Jiang, Peihua Metal Ions Activate the Human Taste Receptor TAS2R7 |
title | Metal Ions Activate the Human Taste Receptor TAS2R7 |
title_full | Metal Ions Activate the Human Taste Receptor TAS2R7 |
title_fullStr | Metal Ions Activate the Human Taste Receptor TAS2R7 |
title_full_unstemmed | Metal Ions Activate the Human Taste Receptor TAS2R7 |
title_short | Metal Ions Activate the Human Taste Receptor TAS2R7 |
title_sort | metal ions activate the human taste receptor tas2r7 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538953/ https://www.ncbi.nlm.nih.gov/pubmed/31066447 http://dx.doi.org/10.1093/chemse/bjz024 |
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