Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins

The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bo...

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Detalles Bibliográficos
Autores principales: Hatlem, Daniel, Trunk, Thomas, Linke, Dirk, Leo, Jack C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539128/
https://www.ncbi.nlm.nih.gov/pubmed/31052154
http://dx.doi.org/10.3390/ijms20092129

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539128/
https://www.ncbi.nlm.nih.gov/pubmed/31052154
http://dx.doi.org/10.3390/ijms20092129

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