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Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method

Safranal, the main volatile chemical of Saffron (Crocus sativus) was studied to estimate its allelopathic effects on the photosynthetic pigment chlorophyll, leaf electrolyte leakage, fresh weight, catalase (CAT), and peroxidase (POX) activity of the test plant Lettuce (Lactuca sativa). In this study...

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Autores principales: Mardani, Hossein, Maninang, John, Appiah, Kwame Sarpong, Oikawa, Yosei, Azizi, Majid, Fujii, Yoshiharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539543/
https://www.ncbi.nlm.nih.gov/pubmed/31072064
http://dx.doi.org/10.3390/molecules24091788
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author Mardani, Hossein
Maninang, John
Appiah, Kwame Sarpong
Oikawa, Yosei
Azizi, Majid
Fujii, Yoshiharu
author_facet Mardani, Hossein
Maninang, John
Appiah, Kwame Sarpong
Oikawa, Yosei
Azizi, Majid
Fujii, Yoshiharu
author_sort Mardani, Hossein
collection PubMed
description Safranal, the main volatile chemical of Saffron (Crocus sativus) was studied to estimate its allelopathic effects on the photosynthetic pigment chlorophyll, leaf electrolyte leakage, fresh weight, catalase (CAT), and peroxidase (POX) activity of the test plant Lettuce (Lactuca sativa). In this study, the effective concentration (EC(50)) of safranal on CAT was estimated to be 6.12 µg/cm(3). CAT activity was inhibited in a dose-dependent manner by the increase in the safranal concentration while POX activity was increased. Moreover, Safranal caused significant physiological changes in chlorophyll content, leaf electrolyte leakage, and fresh weight of several weed species with Lolium multiflorum being the most sensitive. Furthermore, 5 µM Safranal showed significant inhibitory activity against dicotyledonous in comparison to the monocotyledons under greenhouse conditions. The inhibition of the CAT by safranal was similar to those of uncompetitive inhibitors, and therefore the decline in carbon fixation by plants might be the mechanism behind the inhibitory activity of safranal.
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spelling pubmed-65395432019-05-31 Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method Mardani, Hossein Maninang, John Appiah, Kwame Sarpong Oikawa, Yosei Azizi, Majid Fujii, Yoshiharu Molecules Article Safranal, the main volatile chemical of Saffron (Crocus sativus) was studied to estimate its allelopathic effects on the photosynthetic pigment chlorophyll, leaf electrolyte leakage, fresh weight, catalase (CAT), and peroxidase (POX) activity of the test plant Lettuce (Lactuca sativa). In this study, the effective concentration (EC(50)) of safranal on CAT was estimated to be 6.12 µg/cm(3). CAT activity was inhibited in a dose-dependent manner by the increase in the safranal concentration while POX activity was increased. Moreover, Safranal caused significant physiological changes in chlorophyll content, leaf electrolyte leakage, and fresh weight of several weed species with Lolium multiflorum being the most sensitive. Furthermore, 5 µM Safranal showed significant inhibitory activity against dicotyledonous in comparison to the monocotyledons under greenhouse conditions. The inhibition of the CAT by safranal was similar to those of uncompetitive inhibitors, and therefore the decline in carbon fixation by plants might be the mechanism behind the inhibitory activity of safranal. MDPI 2019-05-08 /pmc/articles/PMC6539543/ /pubmed/31072064 http://dx.doi.org/10.3390/molecules24091788 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mardani, Hossein
Maninang, John
Appiah, Kwame Sarpong
Oikawa, Yosei
Azizi, Majid
Fujii, Yoshiharu
Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method
title Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method
title_full Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method
title_fullStr Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method
title_full_unstemmed Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method
title_short Evaluation of Biological Response of Lettuce (Lactuca sativa L.) and Weeds to Safranal Allelochemical of Saffron (Crocus sativus) by Using Static Exposure Method
title_sort evaluation of biological response of lettuce (lactuca sativa l.) and weeds to safranal allelochemical of saffron (crocus sativus) by using static exposure method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539543/
https://www.ncbi.nlm.nih.gov/pubmed/31072064
http://dx.doi.org/10.3390/molecules24091788
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