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Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation
The effects of new strains of lactic acid bacteria on alfalfa silage fermentation were evaluated. The experiment was performed using a completely randomized design (with three replicates) based on a 6 × 6 factorial assay with 6 inoculants (I): Control (CTRL), Commercial inoculant (CI), Lactobacillus...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541639/ https://www.ncbi.nlm.nih.gov/pubmed/31142784 http://dx.doi.org/10.1038/s41598-019-44520-9 |
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author | Nascimento Agarussi, Mariele Cristina Gomes Pereira, Odilon Paula, Rosinea Aparecida de Silva, Vanessa Paula da Santos Roseira, João Paulo Fonseca e Silva, Fabyano |
author_facet | Nascimento Agarussi, Mariele Cristina Gomes Pereira, Odilon Paula, Rosinea Aparecida de Silva, Vanessa Paula da Santos Roseira, João Paulo Fonseca e Silva, Fabyano |
author_sort | Nascimento Agarussi, Mariele Cristina |
collection | PubMed |
description | The effects of new strains of lactic acid bacteria on alfalfa silage fermentation were evaluated. The experiment was performed using a completely randomized design (with three replicates) based on a 6 × 6 factorial assay with 6 inoculants (I): Control (CTRL), Commercial inoculant (CI), Lactobacillus pentosus 14.7SE (LPE), Lactobacillus plantarum 3.7E (LP), Pediococcus pentosaceus 14.15SE (PP), and Lactobacillus plantarum 3.7E + Pediococcus pentosaceus 14.15SE (LP + PP), and six fermentation periods (P): 1, 3, 7, 14, 28 and 56 days. Alfalfa was wilted for 6 h in the field, which increased the dry matter content to 368 g/kg as fed. The CP and yeast population decreased during the fermentation process. Silage inoculated with the PP strain had the lowest pH values beginning at 14 d of fermentation and the lowest acetic acid concentration on the last day of fermentation. New strains more efficiently regulated enterobacteria and mold populations at days 56 and 28, respectively. Silages inoculated with the PP strain had a higher coefficient of in vitro dry matter digestibility than LP silages. All of the tested novel strains resulted in positive effects on at least one chemical property of the silage during the fermentation process. However, the adding of P. pentosaceus can be indicated as the better for silage quality considering the tested treatments in the present study. |
format | Online Article Text |
id | pubmed-6541639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65416392019-06-07 Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation Nascimento Agarussi, Mariele Cristina Gomes Pereira, Odilon Paula, Rosinea Aparecida de Silva, Vanessa Paula da Santos Roseira, João Paulo Fonseca e Silva, Fabyano Sci Rep Article The effects of new strains of lactic acid bacteria on alfalfa silage fermentation were evaluated. The experiment was performed using a completely randomized design (with three replicates) based on a 6 × 6 factorial assay with 6 inoculants (I): Control (CTRL), Commercial inoculant (CI), Lactobacillus pentosus 14.7SE (LPE), Lactobacillus plantarum 3.7E (LP), Pediococcus pentosaceus 14.15SE (PP), and Lactobacillus plantarum 3.7E + Pediococcus pentosaceus 14.15SE (LP + PP), and six fermentation periods (P): 1, 3, 7, 14, 28 and 56 days. Alfalfa was wilted for 6 h in the field, which increased the dry matter content to 368 g/kg as fed. The CP and yeast population decreased during the fermentation process. Silage inoculated with the PP strain had the lowest pH values beginning at 14 d of fermentation and the lowest acetic acid concentration on the last day of fermentation. New strains more efficiently regulated enterobacteria and mold populations at days 56 and 28, respectively. Silages inoculated with the PP strain had a higher coefficient of in vitro dry matter digestibility than LP silages. All of the tested novel strains resulted in positive effects on at least one chemical property of the silage during the fermentation process. However, the adding of P. pentosaceus can be indicated as the better for silage quality considering the tested treatments in the present study. Nature Publishing Group UK 2019-05-29 /pmc/articles/PMC6541639/ /pubmed/31142784 http://dx.doi.org/10.1038/s41598-019-44520-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nascimento Agarussi, Mariele Cristina Gomes Pereira, Odilon Paula, Rosinea Aparecida de Silva, Vanessa Paula da Santos Roseira, João Paulo Fonseca e Silva, Fabyano Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
title | Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
title_full | Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
title_fullStr | Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
title_full_unstemmed | Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
title_short | Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
title_sort | novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541639/ https://www.ncbi.nlm.nih.gov/pubmed/31142784 http://dx.doi.org/10.1038/s41598-019-44520-9 |
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