The Effectiveness of the Whole Body Cryotherapy Strategies: A Comparison of Different Duration and Temperature on the Antioxidative Status in the Experimental Rat Model

BACKGROUND: We examined the effectiveness of the systemic cryotherapy in terms of the temperature and duration of the therapeutic series measured by oxidative stress markers in the rat animal model. METHODS: Antioxidants in serum, plasma, liver, and erythrocytes were evaluated in two study groups following 1 min exposure to − 60°C and − 90°C, for 5 and 10 days. RESULTS: Superoxide dismutase activity in the tissues was lower than in the serum. The glutathione peroxidase was significantly higher in − 60°C than in − 90°C, in both 5 and 10 days of exposition. The liver catalase CAT were significantly lower in − 60°C when compared to − 90°C for 5 and 10 sessions of exposure. In all analysed tissues, the sessions of cryotherapy, – 60/5 and – 60/10, were more effective in reduction malondialdehyde than sessions of − 90/5 and − 90/10. The highest total antioxidant capacity was observed in the − 60/5 group. CONCLUSIONS: Whole body cryotherapy based on temperature − 60°C may be considered as more beneficial than − 90°C for most of the oxidative stress (OS) markers measured in the selected tissues. The temp. − 60°C is more beneficial than − 90°C when measured by activity of Total SOD, CAT, and GPx. The therapeutic sessions − 60/10 and − 60/5 were the optimal schemes of WBC model in terms of TAC and MDA amount.