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Immunocapture of virions with virus-specific antibodies prior to high-throughput sequencing effectively enriches for virus-specific sequences

Virus discovery based on high-throughput sequencing relies on enrichment for virus sequences prior to library preparation to achieve a sufficient number of viral reads. In general, preparations of double-stranded RNA or total RNA preparations treated to remove rRNA are used for sequence enrichment....

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Detalles Bibliográficos
Autores principales: Knierim, Dennis, Menzel, Wulf, Winter, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542260/
https://www.ncbi.nlm.nih.gov/pubmed/31071169
http://dx.doi.org/10.1371/journal.pone.0216713