Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing

cDNA library preparation is important for many high-throughput sequencing applications, such as RNA G-quadruplex structure sequencing (rG4-seq). A systematic evaluation of the procedures of the experimental pipeline, however, is lacking. Herein, we perform a comprehensive assessment of the 5 key exp...

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Detalles Bibliográficos
Autores principales: Yeung, Pui Yan, Zhao, Jieyu, Chow, Eugene Yui-Ching, Mou, Xi, Hong, HuiQi, Chen, Leilei, Chan, Ting-Fung, Kwok, Chun Kit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542847/
https://www.ncbi.nlm.nih.gov/pubmed/31147619
http://dx.doi.org/10.1038/s41598-019-44541-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542847/
https://www.ncbi.nlm.nih.gov/pubmed/31147619
http://dx.doi.org/10.1038/s41598-019-44541-4

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