Optimizing locked nucleic acid/2’-O-methyl-RNA fluorescence in situ hybridization (LNA/2’OMe-FISH) procedure for bacterial detection

Despite the successful application of LNA/2’OMe-FISH procedures for bacteria detection, there is a lack of knowledge on the properties that affect hybridization. Such information is crucial for the rational design of protocols. Hence, this work aimed to evaluate the effect of three essential factors...

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Detalles Bibliográficos
Autores principales: Azevedo, Andreia S., Sousa, Inês M., Fernandes, Ricardo M., Azevedo, Nuno F., Almeida, Carina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544301/
https://www.ncbi.nlm.nih.gov/pubmed/31150460
http://dx.doi.org/10.1371/journal.pone.0217689

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544301/
https://www.ncbi.nlm.nih.gov/pubmed/31150460
http://dx.doi.org/10.1371/journal.pone.0217689

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