Antibody-targeted chromatin enables effective intracellular delivery and functionality of CRISPR/Cas9 expression plasmids

We report a novel system for efficient and specific targeted delivery of large nucleic acids to and into cells. Plasmid DNA and core histones were assembled to chromatin by salt gradient dialysis and subsequently connected to bispecific antibody derivatives (bsAbs) via a nucleic acid binding peptide...

Descripción completa

Detalles Bibliográficos
Autores principales: Killian, Tobias, Buntz, Annette, Herlet, Teresa, Seul, Heike, Mundigl, Olaf, Längst, Gernot, Brinkmann, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547418/
https://www.ncbi.nlm.nih.gov/pubmed/30809660
http://dx.doi.org/10.1093/nar/gkz137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547418/
https://www.ncbi.nlm.nih.gov/pubmed/30809660
http://dx.doi.org/10.1093/nar/gkz137

Ejemplares similares