Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells

FRET (Fluorescence Resonance Energy Transfer) measurements are commonly applied to proof protein-protein interactions. However, standard methods of live cell FRET microscopy and signal normalization only allow a principle assessment of mutual binding and are unable to deduce quantitative information...

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Detalles Bibliográficos
Autores principales: Hochreiter, Bernhard, Kunze, Markus, Moser, Bernhard, Schmid, Johannes A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547726/
https://www.ncbi.nlm.nih.gov/pubmed/31160659
http://dx.doi.org/10.1038/s41598-019-44650-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547726/
https://www.ncbi.nlm.nih.gov/pubmed/31160659
http://dx.doi.org/10.1038/s41598-019-44650-0

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