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Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells
FRET (Fluorescence Resonance Energy Transfer) measurements are commonly applied to proof protein-protein interactions. However, standard methods of live cell FRET microscopy and signal normalization only allow a principle assessment of mutual binding and are unable to deduce quantitative information...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547726/ https://www.ncbi.nlm.nih.gov/pubmed/31160659 http://dx.doi.org/10.1038/s41598-019-44650-0 |