Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor

Cleavage of affinity tags by specific proteases can be exploited for highly selective affinity chromatography. The SUMO/SENP1 system is the most efficient for such application but fails in eukaryotic expression because it cross-reacts with endogenous proteases. Using a novel selection system, we hav...

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Detalles Bibliográficos
Autores principales: Vera Rodriguez, Arturo, Frey, Steffen, Görlich, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548132/
https://www.ncbi.nlm.nih.gov/pubmed/31023724
http://dx.doi.org/10.1083/jcb.201812091

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548132/
https://www.ncbi.nlm.nih.gov/pubmed/31023724
http://dx.doi.org/10.1083/jcb.201812091

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