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Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor
Cleavage of affinity tags by specific proteases can be exploited for highly selective affinity chromatography. The SUMO/SENP1 system is the most efficient for such application but fails in eukaryotic expression because it cross-reacts with endogenous proteases. Using a novel selection system, we hav...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Rockefeller University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548132/ https://www.ncbi.nlm.nih.gov/pubmed/31023724 http://dx.doi.org/10.1083/jcb.201812091 |