iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions

Staphylococcus aureus (S. aureus) is an extremely halotolerant pathogenic bacterium with high osmotic stress tolerance, and it is frequently encountered in aquatic production and preservation. However, the mechanism underlying the extremely high osmotic stress tolerance of S. aureus remains unclear....

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Autores principales: Ming, Tinghong, Geng, Lingxin, Feng, Ying, Lu, Chenyang, Zhou, Jun, Li, Yanyan, Zhang, Dijun, He, Shan, Li, Ye, Cheong, Lingzhi, Su, Xiurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549500/
https://www.ncbi.nlm.nih.gov/pubmed/31191466
http://dx.doi.org/10.3389/fmicb.2019.01082
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author Ming, Tinghong
Geng, Lingxin
Feng, Ying
Lu, Chenyang
Zhou, Jun
Li, Yanyan
Zhang, Dijun
He, Shan
Li, Ye
Cheong, Lingzhi
Su, Xiurong
author_facet Ming, Tinghong
Geng, Lingxin
Feng, Ying
Lu, Chenyang
Zhou, Jun
Li, Yanyan
Zhang, Dijun
He, Shan
Li, Ye
Cheong, Lingzhi
Su, Xiurong
author_sort Ming, Tinghong
collection PubMed
description Staphylococcus aureus (S. aureus) is an extremely halotolerant pathogenic bacterium with high osmotic stress tolerance, and it is frequently encountered in aquatic production and preservation. However, the mechanism underlying the extremely high osmotic stress tolerance of S. aureus remains unclear. In this study, the isobaric tags for relative and absolute quantification (iTRAQ) method was used to identify the differentially expressed proteins (DEPs) under different sodium chloride (NaCl) concentrations. Compared with the control group (0% NaCl), the 10 and 20% NaCl groups had 484 DEPs and 750 DEPs, respectively. Compared with the 10% NaCl group, the 20% NaCl group had 361 DEPs. Among the DEPs, proteins involved in fatty acid synthesis, proline/glycine betaine biosynthesis and transportation, stress tolerance, cell wall biosynthesis and the TCA cycle were upregulated, whereas proteins associated with biofilm formation and pathogenic infections were downregulated. The results obtained in this study indicate that under extremely high osmotic stress, modification of the cell membrane structure, increased biosynthesis and transportation of osmotic protectants, and redistribution of energy metabolism contribute to the osmotic stress tolerance of S. aureus, and the infectious ability of the bacteria may be limited. The aim of this study was to provide new insight into how S. aureus tolerates the high-salt conditions involved in aquatic production and preservation.
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spelling pubmed-65495002019-06-12 iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions Ming, Tinghong Geng, Lingxin Feng, Ying Lu, Chenyang Zhou, Jun Li, Yanyan Zhang, Dijun He, Shan Li, Ye Cheong, Lingzhi Su, Xiurong Front Microbiol Microbiology Staphylococcus aureus (S. aureus) is an extremely halotolerant pathogenic bacterium with high osmotic stress tolerance, and it is frequently encountered in aquatic production and preservation. However, the mechanism underlying the extremely high osmotic stress tolerance of S. aureus remains unclear. In this study, the isobaric tags for relative and absolute quantification (iTRAQ) method was used to identify the differentially expressed proteins (DEPs) under different sodium chloride (NaCl) concentrations. Compared with the control group (0% NaCl), the 10 and 20% NaCl groups had 484 DEPs and 750 DEPs, respectively. Compared with the 10% NaCl group, the 20% NaCl group had 361 DEPs. Among the DEPs, proteins involved in fatty acid synthesis, proline/glycine betaine biosynthesis and transportation, stress tolerance, cell wall biosynthesis and the TCA cycle were upregulated, whereas proteins associated with biofilm formation and pathogenic infections were downregulated. The results obtained in this study indicate that under extremely high osmotic stress, modification of the cell membrane structure, increased biosynthesis and transportation of osmotic protectants, and redistribution of energy metabolism contribute to the osmotic stress tolerance of S. aureus, and the infectious ability of the bacteria may be limited. The aim of this study was to provide new insight into how S. aureus tolerates the high-salt conditions involved in aquatic production and preservation. Frontiers Media S.A. 2019-05-29 /pmc/articles/PMC6549500/ /pubmed/31191466 http://dx.doi.org/10.3389/fmicb.2019.01082 Text en Copyright © 2019 Ming, Geng, Feng, Lu, Zhou, Li, Zhang, He, Li, Cheong and Su. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ming, Tinghong
Geng, Lingxin
Feng, Ying
Lu, Chenyang
Zhou, Jun
Li, Yanyan
Zhang, Dijun
He, Shan
Li, Ye
Cheong, Lingzhi
Su, Xiurong
iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions
title iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions
title_full iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions
title_fullStr iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions
title_full_unstemmed iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions
title_short iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions
title_sort itraq-based quantitative proteomic profiling of staphylococcus aureus under different osmotic stress conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549500/
https://www.ncbi.nlm.nih.gov/pubmed/31191466
http://dx.doi.org/10.3389/fmicb.2019.01082
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