Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments

Microbial communities are commonly studied using culture-independent methods, such as 16S rRNA gene sequencing. However, one challenge in accurately characterizing microbial communities is exogenous bacterial DNA contamination, particularly in low-microbial-biomass niches. Computational approaches t...

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Detalles Bibliográficos
Autores principales: Karstens, Lisa, Asquith, Mark, Davin, Sean, Fair, Damien, Gregory, W. Thomas, Wolfe, Alan J., Braun, Jonathan, McWeeney, Shannon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550369/
https://www.ncbi.nlm.nih.gov/pubmed/31164452
http://dx.doi.org/10.1128/mSystems.00290-19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550369/
https://www.ncbi.nlm.nih.gov/pubmed/31164452
http://dx.doi.org/10.1128/mSystems.00290-19

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