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Label-free and washing-free alkaline phosphatase assay using a personal glucose meter

We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In prin...

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Detalles Bibliográficos
Autores principales: Ahn, Jun Ki, Kim, Hyo Yong, Lee, Chang Yeol, Park, Ki Soo, Park, Hyun Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551882/
https://www.ncbi.nlm.nih.gov/pubmed/31178924
http://dx.doi.org/10.1186/s13036-019-0182-3