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Label-free and washing-free alkaline phosphatase assay using a personal glucose meter
We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In prin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551882/ https://www.ncbi.nlm.nih.gov/pubmed/31178924 http://dx.doi.org/10.1186/s13036-019-0182-3 |