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Label-free and washing-free alkaline phosphatase assay using a personal glucose meter
We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In prin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551882/ https://www.ncbi.nlm.nih.gov/pubmed/31178924 http://dx.doi.org/10.1186/s13036-019-0182-3 |
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author | Ahn, Jun Ki Kim, Hyo Yong Lee, Chang Yeol Park, Ki Soo Park, Hyun Gyu |
author_facet | Ahn, Jun Ki Kim, Hyo Yong Lee, Chang Yeol Park, Ki Soo Park, Hyun Gyu |
author_sort | Ahn, Jun Ki |
collection | PubMed |
description | We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In principle, the presence of target ALP scavenges on adenosine 5′-triphosphate (ATP), a phosphate source for hexokinase-catalyzed reactions, and thus suppresses the ensuing cascade enzymatic reactions. As a result, the initial high amount of glucose is maintained and the amount of glucose, which is proportional to ALP activity, is simply measured by a hand-held PGM. Based on this novel strategy, we successfully determined the ALP activity down to 8.9 U/L with the high selectivity. In addition, the diagnostic capability of this method was demonstrated by reliably assaying the ALP activity in non-diluted human blood without any pretreatment steps. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13036-019-0182-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6551882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65518822019-06-07 Label-free and washing-free alkaline phosphatase assay using a personal glucose meter Ahn, Jun Ki Kim, Hyo Yong Lee, Chang Yeol Park, Ki Soo Park, Hyun Gyu J Biol Eng Letter to the Editor We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In principle, the presence of target ALP scavenges on adenosine 5′-triphosphate (ATP), a phosphate source for hexokinase-catalyzed reactions, and thus suppresses the ensuing cascade enzymatic reactions. As a result, the initial high amount of glucose is maintained and the amount of glucose, which is proportional to ALP activity, is simply measured by a hand-held PGM. Based on this novel strategy, we successfully determined the ALP activity down to 8.9 U/L with the high selectivity. In addition, the diagnostic capability of this method was demonstrated by reliably assaying the ALP activity in non-diluted human blood without any pretreatment steps. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13036-019-0182-3) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-04 /pmc/articles/PMC6551882/ /pubmed/31178924 http://dx.doi.org/10.1186/s13036-019-0182-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Letter to the Editor Ahn, Jun Ki Kim, Hyo Yong Lee, Chang Yeol Park, Ki Soo Park, Hyun Gyu Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
title | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
title_full | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
title_fullStr | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
title_full_unstemmed | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
title_short | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
title_sort | label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551882/ https://www.ncbi.nlm.nih.gov/pubmed/31178924 http://dx.doi.org/10.1186/s13036-019-0182-3 |
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