SUN-486 Central Role of Gonadotropin Inducible Ovarian Transcription Factor 1 (Giot1) on Ingestive Behaviour

Giot1 is a kruppel-type zinc finger protein induced by gonadotropin in teca cells of the ovary and also identified in the central nervous system areas related with hydroeletrolitic and energy homeostasis. Osmotic challenges induce simultaneously an increase in the Giot1 and AVP mRNA expressions in the paraventricular nucleus (PVN) of the hypothalamus. Our hypothesis was that Giot1 had a central role on hydromineral and energy balance modulating the ingestive behavior. To confirm this hypothesis, female Sprague-Dawley(200g) rats were maintained on metabolic cages with free access to diet and fluids. Water was offered during 8 days followed by water and 0.3M NaCl solution during 12 days. After these 20 days, animals were randomly assigned into the Giot1 gene knockdown group and submitted to a bilateral microinjection of small hairpin RNA expressing lentiviral vector (shGiot1) into the PVN. shGFP lentivirus was used as control group (Scramble group). After recovery, we evaluated the ingestive behavior during 20 days. The estrous cycle was verified daily along the experiment. This study was conducted following the “Guide for the Care and Use of Laboratory Animals” (NIH Publication nº85-23, 1996). The software STATISTICA 7.0 was used for statistical analysis. The Giot1 knockdown promoted a decrease of salt preference compared with the scramble group (63.37% ± 1.61, n=6 vs. 68.79% ± 2.02, n= 6; p=0.04). On the other hand, the Giot1 knockdown group had a higher food intake compared with the scramble group (12.39g ± 0.22, n= 6 vs. 10.21g ± 0.22, n= 6; p<0.0001). However, the final body weight of shGiot1 group was not significantly different from scramble group (252g ± 2.01, n= 6 vs. 246.1g ± 2.27, n= 6; p= 0,06). There was no difference in the content of white retroperitoneal and brown interscapular adipose tissue between Giot1 knockdown and control groups. It is well known that PVN neurons have a participation modulating locomotor activity, so we can suggest that Giot1 has a direct or indirect effect on central control of locomotor activity too. These data confirmed that Giot1 in PVN modulates salt preference and food intake. However the mechanisms involved in this behaviour remains unclear. Financial Support: FAPESP and CNPq.