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New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
AIM: A rapid UPLC–MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. MATERIALS AND METHODS: The analysis was achieved on a C(18) analytical column, using a gradient elution...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554689/ https://www.ncbi.nlm.nih.gov/pubmed/31245038 http://dx.doi.org/10.2144/fsoa-2018-0113 |
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author | Bobin-Dubigeon, Christine Campone, Mario Rossignol, Elsa Salaun, Estelle Amiand, Marie-Bernadette Bard, Jean-Marie |
author_facet | Bobin-Dubigeon, Christine Campone, Mario Rossignol, Elsa Salaun, Estelle Amiand, Marie-Bernadette Bard, Jean-Marie |
author_sort | Bobin-Dubigeon, Christine |
collection | PubMed |
description | AIM: A rapid UPLC–MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. MATERIALS AND METHODS: The analysis was achieved on a C(18) analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min. RESULTS: The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity. CONCLUSION: The method is applied routinely for TAM monitoring from patients undergoing therapy. |
format | Online Article Text |
id | pubmed-6554689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65546892019-06-26 New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients Bobin-Dubigeon, Christine Campone, Mario Rossignol, Elsa Salaun, Estelle Amiand, Marie-Bernadette Bard, Jean-Marie Future Sci OA Methodology AIM: A rapid UPLC–MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. MATERIALS AND METHODS: The analysis was achieved on a C(18) analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min. RESULTS: The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity. CONCLUSION: The method is applied routinely for TAM monitoring from patients undergoing therapy. Future Science Ltd 2019-03-22 /pmc/articles/PMC6554689/ /pubmed/31245038 http://dx.doi.org/10.2144/fsoa-2018-0113 Text en © 2019 Christine Bobin-Dubigeon This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Methodology Bobin-Dubigeon, Christine Campone, Mario Rossignol, Elsa Salaun, Estelle Amiand, Marie-Bernadette Bard, Jean-Marie New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
title | New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
title_full | New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
title_fullStr | New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
title_full_unstemmed | New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
title_short | New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
title_sort | new uplc–ms/ms assay for the determination of tamoxifen and its metabolites in human plasma, application to patients |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554689/ https://www.ncbi.nlm.nih.gov/pubmed/31245038 http://dx.doi.org/10.2144/fsoa-2018-0113 |
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