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New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients

AIM: A rapid UPLC–MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. MATERIALS AND METHODS: The analysis was achieved on a C(18) analytical column, using a gradient elution...

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Autores principales: Bobin-Dubigeon, Christine, Campone, Mario, Rossignol, Elsa, Salaun, Estelle, Amiand, Marie-Bernadette, Bard, Jean-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554689/
https://www.ncbi.nlm.nih.gov/pubmed/31245038
http://dx.doi.org/10.2144/fsoa-2018-0113
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author Bobin-Dubigeon, Christine
Campone, Mario
Rossignol, Elsa
Salaun, Estelle
Amiand, Marie-Bernadette
Bard, Jean-Marie
author_facet Bobin-Dubigeon, Christine
Campone, Mario
Rossignol, Elsa
Salaun, Estelle
Amiand, Marie-Bernadette
Bard, Jean-Marie
author_sort Bobin-Dubigeon, Christine
collection PubMed
description AIM: A rapid UPLC–MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. MATERIALS AND METHODS: The analysis was achieved on a C(18) analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min. RESULTS: The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity. CONCLUSION: The method is applied routinely for TAM monitoring from patients undergoing therapy.
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spelling pubmed-65546892019-06-26 New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients Bobin-Dubigeon, Christine Campone, Mario Rossignol, Elsa Salaun, Estelle Amiand, Marie-Bernadette Bard, Jean-Marie Future Sci OA Methodology AIM: A rapid UPLC–MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation. MATERIALS AND METHODS: The analysis was achieved on a C(18) analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min. RESULTS: The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity. CONCLUSION: The method is applied routinely for TAM monitoring from patients undergoing therapy. Future Science Ltd 2019-03-22 /pmc/articles/PMC6554689/ /pubmed/31245038 http://dx.doi.org/10.2144/fsoa-2018-0113 Text en © 2019 Christine Bobin-Dubigeon This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Methodology
Bobin-Dubigeon, Christine
Campone, Mario
Rossignol, Elsa
Salaun, Estelle
Amiand, Marie-Bernadette
Bard, Jean-Marie
New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
title New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
title_full New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
title_fullStr New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
title_full_unstemmed New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
title_short New UPLC–MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
title_sort new uplc–ms/ms assay for the determination of tamoxifen and its metabolites in human plasma, application to patients
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554689/
https://www.ncbi.nlm.nih.gov/pubmed/31245038
http://dx.doi.org/10.2144/fsoa-2018-0113
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