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High-throughput identification of dominant negative polypeptides in yeast
Dominant negative polypeptides can inhibit protein function by binding to the wild type version or by titrating a ligand. Here, we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth....
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555411/ https://www.ncbi.nlm.nih.gov/pubmed/30962621 http://dx.doi.org/10.1038/s41592-019-0368-0 |