High-throughput identification of dominant negative polypeptides in yeast

Dominant negative polypeptides can inhibit protein function by binding to the wild type version or by titrating a ligand. Here, we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth....

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Detalles Bibliográficos
Autores principales: Dorrity, Michael W., Queitsch, Christine, Fields, Stanley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555411/
https://www.ncbi.nlm.nih.gov/pubmed/30962621
http://dx.doi.org/10.1038/s41592-019-0368-0
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author Dorrity, Michael W.
Queitsch, Christine
Fields, Stanley
author_facet Dorrity, Michael W.
Queitsch, Christine
Fields, Stanley
author_sort Dorrity, Michael W.
collection PubMed
description Dominant negative polypeptides can inhibit protein function by binding to the wild type version or by titrating a ligand. Here, we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth. The method can uncover numerous inhibitory polypeptides for a protein and thereby define these fragments with exquisite resolution, even pinpointing individual residues with critical functional roles.
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spelling pubmed-65554112019-10-08 High-throughput identification of dominant negative polypeptides in yeast Dorrity, Michael W. Queitsch, Christine Fields, Stanley Nat Methods Article Dominant negative polypeptides can inhibit protein function by binding to the wild type version or by titrating a ligand. Here, we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth. The method can uncover numerous inhibitory polypeptides for a protein and thereby define these fragments with exquisite resolution, even pinpointing individual residues with critical functional roles. 2019-04-08 2019-05 /pmc/articles/PMC6555411/ /pubmed/30962621 http://dx.doi.org/10.1038/s41592-019-0368-0 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Dorrity, Michael W.
Queitsch, Christine
Fields, Stanley
High-throughput identification of dominant negative polypeptides in yeast
title High-throughput identification of dominant negative polypeptides in yeast
title_full High-throughput identification of dominant negative polypeptides in yeast
title_fullStr High-throughput identification of dominant negative polypeptides in yeast
title_full_unstemmed High-throughput identification of dominant negative polypeptides in yeast
title_short High-throughput identification of dominant negative polypeptides in yeast
title_sort high-throughput identification of dominant negative polypeptides in yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555411/
https://www.ncbi.nlm.nih.gov/pubmed/30962621
http://dx.doi.org/10.1038/s41592-019-0368-0
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