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A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we com...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556159/ https://www.ncbi.nlm.nih.gov/pubmed/30887273 http://dx.doi.org/10.1007/s10561-019-09751-0 |