A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria

Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we com...

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Detalles Bibliográficos
Autores principales: Liu, Baoyan, Lu, Yanqin, Wang, Yong, Ge, Luna, Zhai, Naixiang, Han, Jinxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Full Length Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556159/
https://www.ncbi.nlm.nih.gov/pubmed/30887273
http://dx.doi.org/10.1007/s10561-019-09751-0
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author Liu, Baoyan
Lu, Yanqin
Wang, Yong
Ge, Luna
Zhai, Naixiang
Han, Jinxiang
author_facet Liu, Baoyan
Lu, Yanqin
Wang, Yong
Ge, Luna
Zhai, Naixiang
Han, Jinxiang
author_sort Liu, Baoyan
collection PubMed
description Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.
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spelling pubmed-65561592019-06-21 A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria Liu, Baoyan Lu, Yanqin Wang, Yong Ge, Luna Zhai, Naixiang Han, Jinxiang Cell Tissue Bank Full Length Paper Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests. Springer Netherlands 2019-03-18 2019 /pmc/articles/PMC6556159/ /pubmed/30887273 http://dx.doi.org/10.1007/s10561-019-09751-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Full Length Paper
Liu, Baoyan
Lu, Yanqin
Wang, Yong
Ge, Luna
Zhai, Naixiang
Han, Jinxiang
A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
title A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
title_full A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
title_fullStr A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
title_full_unstemmed A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
title_short A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
title_sort protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
topic Full Length Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556159/
https://www.ncbi.nlm.nih.gov/pubmed/30887273
http://dx.doi.org/10.1007/s10561-019-09751-0
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