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Development of a markerless gene deletion strategy using rpsL as a counterselectable marker and characterization of the function of RA0C_1534 in Riemerella anatipestifer ATCC11845 using this strategy

Riemerella anatipestifer is a gram-negative bacterium that mainly infects ducks, turkeys and other birds. In a previous study, we established a markerless mutation system based on the pheS mutant as a counterselectable marker. However, the toxic effect of p-Cl-Phe on the R. anatipestifer strain expr...

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Detalles Bibliográficos
Autores principales: Liu, MaFeng, Tian, Xiu, Wang, MengYi, Zhu, DeKang, Wang, MingShu, Jia, RenYong, Chen, Shun, Zhao, XinXin, Yang, Qiao, Wu, Ying, Zhang, ShaQiu, Huang, Juan, Tian, Bin, Chen, XiaoYue, Liu, YunYa, Zhang, Ling, Yu, YanLing, Biville, Francis, Pan, LeiChang, Rehman, Mujeeb Ur, Cheng, AnChun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557517/
https://www.ncbi.nlm.nih.gov/pubmed/31181133
http://dx.doi.org/10.1371/journal.pone.0218241