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Improved method for isolating high-quality RNA from mouse bone with RNAlater at room temperature

Accurate gene expression analysis of bone requires the ability to isolate RNA of good quality. Isolation of intact RNA from frozen bone tissue is problematic since RNA rapidly becomes degraded after thawing. Since we are interested in assessing gene expression from both bone marrow and mineralized b...

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Detalles Bibliográficos
Autores principales: Pedersen, Kim B., Williams, Ashlee, Watt, James, Ronis, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558217/
https://www.ncbi.nlm.nih.gov/pubmed/31198821
http://dx.doi.org/10.1016/j.bonr.2019.100211