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Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid
BACKGROUND: The methods routinely used to detect trichomonads in the lungs are not sensitive enough, and an effective method is urgently needed. METHOD: Primers were first designed to match the conserved area of the 18S rRNA gene of trichomonads. Then, nested PCR was carried out to detect trichomona...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558733/ https://www.ncbi.nlm.nih.gov/pubmed/31182037 http://dx.doi.org/10.1186/s12879-019-4118-9 |