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Engineered cyclodextrin glucanotransferases from Bacillus sp. G‐825‐6 produce large‐ring cyclodextrins with high specificity

Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch. A CGTase from Bacillus sp. G‐825‐6 was engineered by site‐directed mutagenesis at two positions by the construction of the variants Y183W, Y183R, D358R, Y183W/D358R, and Y183R/D358R. Among...

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Detalles Bibliográficos
Autores principales: Sonnendecker, Christian, Melzer, Susanne, Zimmermann, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562119/
https://www.ncbi.nlm.nih.gov/pubmed/30358941
http://dx.doi.org/10.1002/mbo3.757