Cargando…

Isolation and Characterization of Lactobacillus brevis Phages

Lactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potentia...

Descripción completa

Detalles Bibliográficos
Autores principales: Feyereisen, Marine, Mahony, Jennifer, Lugli, Gabriele A., Ventura, Marco, Neve, Horst, Franz, Charles M. A. P., Noben, Jean-Paul, O’Sullivan, Tadhg, van Sinderen, Douwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563214/
https://www.ncbi.nlm.nih.gov/pubmed/31035495
http://dx.doi.org/10.3390/v11050393
_version_ 1783426499037102080
author Feyereisen, Marine
Mahony, Jennifer
Lugli, Gabriele A.
Ventura, Marco
Neve, Horst
Franz, Charles M. A. P.
Noben, Jean-Paul
O’Sullivan, Tadhg
van Sinderen, Douwe
author_facet Feyereisen, Marine
Mahony, Jennifer
Lugli, Gabriele A.
Ventura, Marco
Neve, Horst
Franz, Charles M. A. P.
Noben, Jean-Paul
O’Sullivan, Tadhg
van Sinderen, Douwe
author_sort Feyereisen, Marine
collection PubMed
description Lactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potential to control such spoilage bacteria. Just a few studies on phages infecting Lactobacillus brevis have been performed to date and in the present study, we report the isolation and characterization of five virulent phages capable of infecting Lb. brevis strains. The analysis reveals a high diversity among the isolates, with members belonging to both, the Myoviridae and Siphoviridae families. One isolate, designated phage 3-521, possesses a genome of 140.8 kb, thus representing the largest Lb. brevis phage genome sequenced to date. While the isolated phages do not propagate on Lb. brevis beer-spoiling strains, phages showed activity against these strains, impairing the growth of some Lb. brevis strains. The results highlight the potential of bacteriophage-based treatments as an effective approach to prevent bacterial spoilage of beer.
format Online
Article
Text
id pubmed-6563214
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65632142019-06-17 Isolation and Characterization of Lactobacillus brevis Phages Feyereisen, Marine Mahony, Jennifer Lugli, Gabriele A. Ventura, Marco Neve, Horst Franz, Charles M. A. P. Noben, Jean-Paul O’Sullivan, Tadhg van Sinderen, Douwe Viruses Article Lactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potential to control such spoilage bacteria. Just a few studies on phages infecting Lactobacillus brevis have been performed to date and in the present study, we report the isolation and characterization of five virulent phages capable of infecting Lb. brevis strains. The analysis reveals a high diversity among the isolates, with members belonging to both, the Myoviridae and Siphoviridae families. One isolate, designated phage 3-521, possesses a genome of 140.8 kb, thus representing the largest Lb. brevis phage genome sequenced to date. While the isolated phages do not propagate on Lb. brevis beer-spoiling strains, phages showed activity against these strains, impairing the growth of some Lb. brevis strains. The results highlight the potential of bacteriophage-based treatments as an effective approach to prevent bacterial spoilage of beer. MDPI 2019-04-26 /pmc/articles/PMC6563214/ /pubmed/31035495 http://dx.doi.org/10.3390/v11050393 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feyereisen, Marine
Mahony, Jennifer
Lugli, Gabriele A.
Ventura, Marco
Neve, Horst
Franz, Charles M. A. P.
Noben, Jean-Paul
O’Sullivan, Tadhg
van Sinderen, Douwe
Isolation and Characterization of Lactobacillus brevis Phages
title Isolation and Characterization of Lactobacillus brevis Phages
title_full Isolation and Characterization of Lactobacillus brevis Phages
title_fullStr Isolation and Characterization of Lactobacillus brevis Phages
title_full_unstemmed Isolation and Characterization of Lactobacillus brevis Phages
title_short Isolation and Characterization of Lactobacillus brevis Phages
title_sort isolation and characterization of lactobacillus brevis phages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563214/
https://www.ncbi.nlm.nih.gov/pubmed/31035495
http://dx.doi.org/10.3390/v11050393
work_keys_str_mv AT feyereisenmarine isolationandcharacterizationoflactobacillusbrevisphages
AT mahonyjennifer isolationandcharacterizationoflactobacillusbrevisphages
AT lugligabrielea isolationandcharacterizationoflactobacillusbrevisphages
AT venturamarco isolationandcharacterizationoflactobacillusbrevisphages
AT nevehorst isolationandcharacterizationoflactobacillusbrevisphages
AT franzcharlesmap isolationandcharacterizationoflactobacillusbrevisphages
AT nobenjeanpaul isolationandcharacterizationoflactobacillusbrevisphages
AT osullivantadhg isolationandcharacterizationoflactobacillusbrevisphages
AT vansinderendouwe isolationandcharacterizationoflactobacillusbrevisphages