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Mismatch amplification mutation assay-polymerase chain reaction: A method of detecting fluoroquinolone resistance mechanism in bacterial pathogens

The mismatch amplification assay is a modified version of polymerase chain reaction (PCR) that permits specific amplification of gene sequences with single base pair change. The basis of the technique relies on primer designing. The single nucleotide mismatch at the 3’ proximity of the reverse oligo...

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Detalles Bibliográficos
Autores principales: Deekshit, Vijaya Kumar, Jazeela, Kadeeja, Chakraborty, Gunimala, Rohit, Anusha, Chakraborty, Anirban, Karunasagar, Indrani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563742/
https://www.ncbi.nlm.nih.gov/pubmed/31219078
http://dx.doi.org/10.4103/ijmr.IJMR_2091_17