Cargando…

Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities

Rif1 is a conserved protein regulating replication timing and binds preferentially to the vicinity of late-firing/dormant origins in fission yeast. The Rif1 binding sites on the fission yeast genome have an intrinsic potential to generate G-quadruplex (G4) structures to which purified Rif1 preferent...

Descripción completa

Detalles Bibliográficos
Autores principales: Masai, Hisao, Fukatsu, Rino, Kakusho, Naoko, Kanoh, Yutaka, Moriyama, Kenji, Ma, Yue, Iida, Keisuke, Nagasawa, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565636/
https://www.ncbi.nlm.nih.gov/pubmed/31197198
http://dx.doi.org/10.1038/s41598-019-44736-9
_version_ 1783426687151636480
author Masai, Hisao
Fukatsu, Rino
Kakusho, Naoko
Kanoh, Yutaka
Moriyama, Kenji
Ma, Yue
Iida, Keisuke
Nagasawa, Kazuo
author_facet Masai, Hisao
Fukatsu, Rino
Kakusho, Naoko
Kanoh, Yutaka
Moriyama, Kenji
Ma, Yue
Iida, Keisuke
Nagasawa, Kazuo
author_sort Masai, Hisao
collection PubMed
description Rif1 is a conserved protein regulating replication timing and binds preferentially to the vicinity of late-firing/dormant origins in fission yeast. The Rif1 binding sites on the fission yeast genome have an intrinsic potential to generate G-quadruplex (G4) structures to which purified Rif1 preferentially binds. We previously proposed that Rif1 generates chromatin architecture that may determine replication timing by facilitating the chromatin loop formation. Here, we conducted detailed biochemical analyses on Rif1 and its G4 binding. Rif1 prefers sequences containing long stretches of guanines and binds preferentially to the multimeric G4 of parallel or hybrid/mix topology. Rif1 forms oligomers and binds simultaneously to multiple G4. We present a model on how Rif1 may facilitate the formation of chromatin architecture through its G4 binding and oligomerization properties.
format Online
Article
Text
id pubmed-6565636
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-65656362019-06-20 Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities Masai, Hisao Fukatsu, Rino Kakusho, Naoko Kanoh, Yutaka Moriyama, Kenji Ma, Yue Iida, Keisuke Nagasawa, Kazuo Sci Rep Article Rif1 is a conserved protein regulating replication timing and binds preferentially to the vicinity of late-firing/dormant origins in fission yeast. The Rif1 binding sites on the fission yeast genome have an intrinsic potential to generate G-quadruplex (G4) structures to which purified Rif1 preferentially binds. We previously proposed that Rif1 generates chromatin architecture that may determine replication timing by facilitating the chromatin loop formation. Here, we conducted detailed biochemical analyses on Rif1 and its G4 binding. Rif1 prefers sequences containing long stretches of guanines and binds preferentially to the multimeric G4 of parallel or hybrid/mix topology. Rif1 forms oligomers and binds simultaneously to multiple G4. We present a model on how Rif1 may facilitate the formation of chromatin architecture through its G4 binding and oligomerization properties. Nature Publishing Group UK 2019-06-13 /pmc/articles/PMC6565636/ /pubmed/31197198 http://dx.doi.org/10.1038/s41598-019-44736-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Masai, Hisao
Fukatsu, Rino
Kakusho, Naoko
Kanoh, Yutaka
Moriyama, Kenji
Ma, Yue
Iida, Keisuke
Nagasawa, Kazuo
Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
title Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
title_full Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
title_fullStr Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
title_full_unstemmed Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
title_short Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
title_sort rif1 promotes association of g-quadruplex (g4) by its specific g4 binding and oligomerization activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565636/
https://www.ncbi.nlm.nih.gov/pubmed/31197198
http://dx.doi.org/10.1038/s41598-019-44736-9
work_keys_str_mv AT masaihisao rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT fukatsurino rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT kakushonaoko rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT kanohyutaka rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT moriyamakenji rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT mayue rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT iidakeisuke rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities
AT nagasawakazuo rif1promotesassociationofgquadruplexg4byitsspecificg4bindingandoligomerizationactivities