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Widely applicable background depletion step enables transaminase evolution through solid-phase screening

Directed evolution of transaminases is a widespread technique in the development of highly sought-after biocatalysts for industrial applications. This process, however, is challenged by the limited availability of effective high-throughput protocols to evaluate mutant libraries. Here we report a rap...

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Detalles Bibliográficos
Autores principales: Planchestainer, Matteo, Hegarty, Eimear, Heckmann, Christian M., Gourlay, Louise J., Paradisi, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566068/
https://www.ncbi.nlm.nih.gov/pubmed/31360401
http://dx.doi.org/10.1039/c8sc05712e