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Laboratory-Scale Isolation of Insect Antifreeze Protein for Cryobiology

Micromolar concentrations of hyperactive antifreeze proteins (AFPs) from insects can prevent aqueous solutions from freezing down to at least −6 °C. To explore cryopreservation of cells, tissues and organs at these temperatures without ice formation, we have developed a protocol to reliably produce...

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Detalles Bibliográficos
Autores principales: Tomalty, Heather E., Graham, Laurie A., Eves, Robert, Gruneberg, Audrey K., Davies, Peter L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572240/
https://www.ncbi.nlm.nih.gov/pubmed/31075842
http://dx.doi.org/10.3390/biom9050180